Eugene J. Leys, PhD
PhD
Baylor College of Medicine, 1983
Postdoctoral Training
The Carnegie Institution of Washington
Molecular and population genetics of periodontitis-associated and other oral bacteria
We use an integrated approach that utilizes molecular biology, microbiology and epidemiology to study the molecular and population genetics of periodontitis-associated and other oral bacteria. We have developed a system for the detection and identification of bacterial strains (clonal types) of oral bacteria. By using PCR with species-specific primers, bacteria are detected directly from oral samples without the need for culturing. PCR products are sequenced to determine the genetic identity for individual clonal types and phylogenetic relationships among strains.
Single cell genomics of uncultured, health-associated Tannerella BU063 (oral taxon 286) and comparison to the closely related pathogen Tannerella forsythia Beall, C. J., Campbell, A. G., Dayeh, D. M., Griffen, A. L., Podar, M. & Leys, E. J. Feb 14 2014 In : PLoS ONE. 9, 2, e89398
Diversity and genomic insights into the uncultured Chloroflexi from the human microbiota Campbell, A. G., Schwientek, P., Vishnivetskaya, T., Woyke, T., Levy, S., Beall, C. J., Griffen, A., Leys, E. & Podar, M. Jan 1 2014 In : Environmental Microbiology. 16, 9, p. 2635-2643 9 p.
Multiple Single-Cell Genomes Provide Insight into Functions of Uncultured Deltaproteobacteria in the Human Oral Cavity. Campbell, A. G., Campbell, J. H., Schwientek, P., Woyke, T., Sczyrba, A., Allman, S., Beall, C. J., Griffen, A., Leys, E. & Podar, M. Mar 26 2013 In : PLoS ONE. 8, 3, e59361
Beyond Streptococcus mutans: Dental Caries Onset Linked to Multiple Species by 16S rRNA Community Analysis. Gross, E. L., Beall, C. J., Kutsch, S. R., Firestone, N. D., Leys, E. J. & Griffen, A. L. Oct 16 2012 In : PLoS ONE. 7, 10, e47722
The Drosophila melanogaster host model. Igboin, C. O., Griffen, A. L. & Leys, E. J. Aug 14 2012 In : Journal of Oral Microbiology. 4, 2012, 10368
Distinct and complex bacterial profiles in human periodontitis and health revealed by 16S pyrosequencing. Griffen, A. L., Beall, C. J., Campbell, J. H., Firestone, N. D., Kumar, P. S., Yang, Z. K., Podar, M. & Leys, E. J. Jun 1 2012 In : ISME Journal. 6, 6, p. 1176-1185 10 p.
Filifactor alocis interactions with gingival epithelial cells. Moffatt, C. E., Whitmore, S. E., Griffen, A. L., Leys, E. J. & Lamont, R. J. Dec 1 2011 In : Molecular Oral Microbiology. 26, 6, p. 365-373 9 p.
CORE: A phylogenetically-curated 16S rDNA database of the core oral microbiome. Griffen, A. L., Beall, C. J., Firestone, N. D., Gross, E. L., DiFranco, J. M., Hardman, J. H., Vriesendorp, B., Faust, R. A., Janies, D. A. & Leys, E. J. May 4 2011 In : PLoS ONE. 6, 4, e19051
Porphyromonas gingivalis-host interactions in a drosophila melanogaster model. Igboin, C. O., Tordoff, K. P., Moeschberger, M. L., Griffen, A. L. & Leys, E. J. Jan 1 2011 In : Infection and Immunity. 79, 1, p. 449-458 10 p.
Porphyromonas gingivalis virulence in a drosophila melanogaster model. Igboin, C. O., Moeschberger, M. L., Griffen, A. L. & Leys, E. J. Jan 1 2011 In : Infection and Immunity. 79, 1, p. 439-448 10 p.
Bacterial 16S sequence analysis of severe caries in young permanent teeth. Gross, E. L., Leys, E. J., Gasparovich, S. R., Firestone, N. D., Schwartzbaum, J. A., Janies, D. A., Asnani, K. & Griffen, A. L. Nov 1 2010 In : Journal of Clinical Microbiology. 48, 11, p. 4121-4128 8 p.
Porphyromonas gingivalis strain diversity. Igboin, C. O., Griffen, A. L. & Leys, E. J. Oct 1 2009 In : Journal of Clinical Microbiology. 47, 10, p. 3073-3081 9 p.
3185 Postle Hall
305 W. 12th Ave.
Columbus, OH 43210
United States